Table 2.
Mass contents of phospholipid molecular species in the DRG of wild-type and apolipoprotein E knockout mice at 4 months of agea
| |
Molecular species |
WT mice |
ApoE KO mice |
|---|---|---|---|
| [M - H]− | Phosphatidic acid (GPA) | ||
| 671.5 | 16:0−18:2 GPA | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 673.5 | 16:0−18:1 GPA | 0.2 ± 0.1 | 0.2 ± 0.1 |
| 699.5 | 18:1−18:1 GPA | 0.6 ± 0.1 | 0.5 ± 0.1 |
| 701.5 | 18:0−18:1 GPA | 2.2 ± 0.2 | 1.9 ± 0.2 |
| Total GPA | 3.2 ± 0.2 | 3.0 ± 0.3 |
| [M - H]− | Phosphatidylglycerol (GPGro) | ||
|---|---|---|---|
| 745.5 | 16:0−18:2 GPGro | 0.1 ± 0.1 | 0.1 ± 0.1 |
| 747.5 | 16:0−18:1 GPGro | 0.5 ± 0.1 | 0.5 ± 0.1 |
| 771.5 | 18:1−18:2 GPGro | 0.3 ± 0.1 | 0.2 ± 0.1 |
| 773.5 | 18:1−18:1 GPGro | 0.2 ± 0.1 | 0.2 ± 0.1 |
| 775.5 | 18:0−18:2 GPGro | DA | DA |
| Total GPGro | 1.0 ± 0.1 | 1.0 ± 0.1 |
| [M - H]− | Serine glycerophospholipid (GPSer) | ||
|---|---|---|---|
| 758.5 | 16:0−18:2 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 760.5 | 16:0−18:1 | 1.8 ± 0.2 | 2.2 ± 0.1 |
| 770.6 | p18:1−18:1 | 0.7 ± 0.2 | 0.9 ± 0.2 |
| 772.6 | p18:0−18:1 | 2.8 ± 0.2 | 2.9 ± 0.1 |
| 774.6 | p18:0−18:0 | 1.8 ± 0.2 | 1.9 ± 0.1 |
| 786.6 | 18:1−18:1 | 16.5 ± 0.3 | 17.2 ± 0.5 |
| 788.6 | 18:0−18:1 | 74.1 ± 0.8 | 71.8 ± 2.8 |
| 810.6 | 18:0−20:4 | 2.5 ± 0.3 | 2.7 ± 0.2 |
| 812.6 | 18:0−20:3 | 1.4 ± 0.1 | 1.5 ± 0.1 |
| 814.6 | 18:0−20:2 | 1.0 ± 0.1 | 1.2 ± 0.2 |
| 816.6 | 18:1−20:0 | 5.6 ± 0.2 | 5.5 ± 0.2 |
| 834.6 | 18:0−22:6 | 2.8 ± 0.3 | 2.4 ± 0.2 |
| 836.6 | 18:0−22:5 | 0.6 ± 0.2 | 0.4 ± 0.1 |
| 842.7 | 18:1−22:1 | 3.4 ± 0.2 | 3.4 ± 0.1 |
| 844.7 | 18:0−22:1 | 11.2 ± 0.4 | 11.4 ± 0.2 |
| 858.7 | a18:0−24:1 | 2.6 ± 0.2 | 2.6 ± 0.1 |
| 870.7 | 18:1−24:1 | 4.2 ± 0.2 | 4.1 ± 0.1 |
| 872.7 | 18:0−24:1 | 15.4 ± 0.5 | 16.4 ± 0.6 |
| Total GPSer | 148.5 ± 1.6 | 148.8 ± 4.5 |
| [M - H]− | Phosphatidylinositol (GPIns) | ||
|---|---|---|---|
| 833.6 | 16:0−18:2 GPIns | 1.5 ± 0.1 | 0.7 ± 0.1 |
| 835.6 | 16:0−18:1 GPIns | 1.2 ± 0.1 | 1.3 ± 0.1 |
| 857.6 | 16:0−20:4 GPIns | 0.8 ± 0.1 | 0.9 ± 0.1 |
| 859.6 | 18:1−18:2 GPIns | 0.8 ± 0.1 | 0.8 ± 0.1 |
| 861.6 | 18:1−18:1 GPIns | 3.2 ± 0.1 | 3.4 ± 0.2 |
| 863.6 | 18:0−18:1 GPIns | 1.8 ± 0.2 | 2.0 ± 0.1 |
| 883.6 | 18:1−20:4 GPIns | 0.4 ± 0.1 | 0.4 ± 0.1 |
| 885.6 | 18:0−20:4 GPIns | 4.7 ± 0.6 | 5.7 ± 0.2 |
| 887.6 | 18:0−20:3 GPIns | 2.8 ± 0.3 | 3.4 ± 0.1 |
| 889.6 | 18:0−20:2 GPIns | 2.5 ± 0.2 | 2.8 ± 0.2 |
| 909.6 | 18:0−22:6 GPIns | 0.4 ± 0.1 | 0.4 ± 0.1 |
| 911.6 | 18:0−22:5 GPIns | 0.1 ± 0.1 | 0.1 ± 0.1 |
| Total GPIns | 20.1 ± 1.4 | 22.8 ± 0.5 |
| [M - H]− | Ethanolamine glycerophospholipid (GPEtn) | ||
|---|---|---|---|
| 646.5 | p16:0−14:0 | 0.1 ± 0.1 | 0.1 ± 0.1 |
| 672.5 | p14:0−18:1/p18:1−14:0/p16:0−16:1 | 1.0 ± 0.1 | 1.0 ± 1.0 |
| 674.5 | p16:0−16:0/p18:0−14:0 | 0.4 ± 0.1 | 0.4 ± 0.0.1 |
| 698.5 | p16:0−18:2/p18:2−16:0/p18:1−16:1 | 4.0 ± 0.2 | 4.0 ± 0.1 |
| 700.5 | p16:0−18:1/p18:1−16:0/p18:0−16:1 | 29.1 ± 1.7 | 28.7 ± 0.7 |
| 714.5 | d16:0−18:2/d16:1−18:1 | 5.5 ± 0.6 | 4.8 ± 0.1 |
| 716.5 | d16:0−18:1 | 7.9 ± 0.5 | 7.7 ± 0.1 |
| 722.5 | p16:0−20:4 | 2.4 ± 0.1 | 2.4 ± 0.1 |
| 724.5 | p18:1−18:2 | 9.9 ± 0.1 | 10.4 ± 0.8 |
| 726.5 | p18:1−18:1/p18:0−18:2/p20:1−16:1 | 93.6 ± 5.5 | 98.3 ± 3.7 |
| 728.5 | p18:0−18:1/p18:1−18:0/p20:1−16:0/p16:0−20:1/p20:0−16:1 | 73.8 ± 2.7 | 72.2 ± 2.1 |
| 738.5 | d16:0−20:4/d18:2−18:2 | 17.5 ± 0.8 | 18.2 ± 0.5 |
| 740.5 | d18:1−18:2/p19:1−18:1/p18:1−19:1 | 4.8 ± 1.0 | 3.7 ± 0.6 |
| 742.5 | d18:1−18:1/p19:0−18:1/p18:1−19:0 | 18.8 ± 1.1 | 19.9 ± 0.6 |
| 744.5 | d18:0−18:1 | 10.4 ± 0.5 | 10.1 ± 0.2 |
| 746.5 | p16:0−22:6 | 1.7 ± 0.1 | 1.6 ± 0.1 |
| 748.5 | p18:1−20:4/p16:0−22:5 | 10.7 ± 0.3 | 10.2 ± 0.7 |
| 750.5 | p18:0−20:4/p16:0−22:4 | 9.6 ± 0.3 | 9.2 ± 0.5 |
| 752.5 | p20:1−18:2 | 2.1 ± 0.1 | 2.2 ± 0.1 |
| 754.5 | p20:1−18:1/p20:0−18:2/p18:0−20:2/p16:0−22:2/d19:1−18:2/D19:2−18:1 | 4.1 ± 0.2 | 4.2 ± 0.2 |
| 756.5 | p20:0−18:1/d19:1−18:1/p18:0−20:1/p20:1−18:0 | 2.2 ± 0.1 | 2.2 ± 0.1 |
| 760.5 | d16:1−22:6 | 0.8 ± 0.1 | 0.8 ± 0.1 |
| 762.5 | d16:0−22:6/d16:1−22:5 | 3.3 ± 0.2 | 3.3 ± 0.2 |
| 764.5 | d16:0−22:5/d18:1−20:4 | 3.2 ± 0.1 | 3.1 ± 0.2 |
| 766.5 | d18:0−20:4 | 8.0 ± 1.2 | 7.3 ± 0.3 |
| 770.5 | p18:2−22:6 | 1.8 ± 0.1 | 1.9 ± 0.1 |
| 772.5 | p18:1−22:6 | 4.6 ± 0.1 | 4.5 ± 0.2 |
| 774.5 | p18:0−22:6 | 11.1 ± 0.8 | 10.2 ± 0.4 |
| 776.5 | p18:0−22:5/p18:1−22:4/p20:1−20:4 | 8.6 ± 0.2 | 8.6 ± 0.4 |
| 778.5 | p18:0−22:4/p20:0−20:4 | 8.5 ± 0.2 | 8.3 ± 0.3 |
| 780.5 | a20:0−20:4/p20:0−20:3/p18:0−22:3/d19:2−20:2 | 1.8 ± 0.2 | 1.8 ± 0.1 |
| 782.5 | p22:1−18:1/p18:1−22:1/d19:1−20:2/d19:2−20:1 | 2.7 ± 0.2 | 2.8 ± 0.1 |
| 784.5 | a18:1−22:1/p18:0−22:1/d19:1−20:1 | 1.8 ± 0.1 | 1.9 ± 0.1 |
| 788.5 | d18:1−22:6 | 1.6 ± 0.1 | 1.4 ± 0.1 |
| 790.5 | d18:0−22:6 | 9.6 ± 1.3 | 7.9 ± 0.6 |
| 792.5 | d18:0−22:5 | 1.4 ± 0.1 | 1.4 ± 0.1 |
| 794.5 | d20:0−20:4/d20:2−20:2/d18:0−22:4 | 2.1 ± 0.2 | 2.2 ± 0.1 |
| 796.5 | d20:1−20:2 | 0.7 ± 0.1 | 0.8 ± 0.1 |
| 798.5 | d20:1−20:1/d20:0−20:2/d18:1−22:1 | 2.0 ± 0.2 | 2.1 ± 0.1 |
| 800.5 | d20:1−20:0 | 1.5 ± 0.1 | 1.4 ± 0.1 |
| 802.5 | d18:0−22:0/p20:0−22:6 | 4.4 ± 0.2 | 4.5 ± 0.2 |
| 804.5 | p20:0−22:5 | 0.6 ± 0.1 | 0.7 ± 0.1 |
| 806.5 | p20:0−22:4 | 0.7 ± 0.1 | 0.7 ± 0.1 |
| Total GPEtn | 390.1 ± 17.6 | 388.6 ± 11.4 | |
| Total plasmalogen | 284.9 ± 11.4 | 286.0 ± 10.6 | |
| Plasmalogen (%) | 73.0 ± 2.2 | 73.6 ± 1.6 |
| [M + Li]+ | Choline glycerophospholipid (GPCho) | ||
|---|---|---|---|
| 614.5 | a12:0−12:0 | 0.5 ± 0.2 | 0.6 ± 0.1 |
| 712.5 | d14:0−16:0 | 3.3 ± 0.2 | 3.5 ± 0.3 |
| 724.5 | p16:0−16:0 | 0.7 ± 0.1 | 1.0 ± 0.3 |
| 726.5 | a16:0−16:0 | 1.0 ± 0.3 | 1.1 ± 0.2 |
| 738.5 | d16:1−16:0 | 8.2 ± 1.0 | 6.3 ± 0.2 |
| 740.5 | d16:0−16:0 | 18.7 ± 1.9 | 16.0 ± 0.3 |
| 750.5 | p16:0−18:1 | 0.6 ± 0.2 | 0.9 ± 0.1 |
| 752.5 | p16:0−18:0 | 2.5 ± 0.5 | 2.9 ± 0.2 |
| 754.5 | a16:0−18:0 | 0.7 ± 0.1 | 0.5 ± 0.1 |
| 764.6 | d16:0−18:2 | 8.9 ± 1.7 | 10.1 ± 0.1 |
| 766.6 | d16:0−18:1 | 61.5 ± 4.6 | 59.2 ± 1.2 |
| 776.6 | p18:1−18:1 | 0.4 ± 0.3 | 0.6 ± 0.3 |
| 778.6 | p18:0−18:1 | 0.9 ± 0.2 | 0.9 ± 0.1 |
| 780.6 | p18:0−18:0 | 2.2 ± 0.2 | 1.9 ± 0.2 |
| 788.6 | d16:0−20:4/d16:1−20:3 | 6.5 ± 0.4 | 5.9 ± 0.1 |
| 790.6 | d18:1−18:2/d16:0−20:3 | 3.2 ± 0.5 | 3.4 ± 0.1 |
| 792.6 | d18:0−18:2/d18:1−18:1 | 8.9 ± 0.8 | 9.4 ± 0.1 |
| 794.6 | d18:0−18:1 | 19.3 ± 1.3 | 19.3 ± 0.2 |
| 812.6 | d16:0−22:6 | 12.6 ± 1.3 | 12.5 ± 0.5 |
| 814.6 | d18:1−20:4/d18:2−20:3 | 2.1 ± 0.4 | 1.4 ± 0.2 |
| 816.6 | d18:0−20:4/d18:2−20:2 | 5.5 ± 0.4 | 5.1 ± 0.3 |
| 818.6 | d18:0−20:3 | 1.2 ± 0.3 | 1.1 ± 0.4 |
| 830.6 | d19:0−20:4 | 0.5 ± 0.1 | 0.5 ± 0.1 |
| 836.6 | d18:2−22:6 | 0.6 ± 0.1 | 0.5 ± 0.1 |
| 838.6 | d18:1−22:6 | 1.4 ± 0.3 | 1.7 ± 0.1 |
| 840.6 | d18:0−22:6 | 3.2 ± 0.3 | 2.6 ± 0.1 |
| 848.6 | d20:1−20:1/d18:0−22:2 | 0.6 ± 0.3 | 0.8 ± 0.1 |
| 850.6 | d20:0−20:1/p20:1−22:6 | 3.4 ± 0.3 | 3.2 ± 0.1 |
| 864.6 | d20:2−22:6 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 876.6 | p22:3−22:5/d20:1−22:1 | 1.2 ± 0.1 | 1.0 ± 0.2 |
| 878.6 | p22:1−22:6/p22:6−22:1/d20:0−22:1 | 4.6 ± 0.6 | 6.1 ± 0.2 |
| Total GPCho | 184.4 ± 5.5 | 180.1 ± 1.5 |
a
Mouse DRG lipids were extracted by a modified Bligh and Dyer procedure and the phospholipid molecular species in the lipid extracts were identified and quantified using a 2D ESI/MS technique as described under Materials and methods. The results are expressed in nmol/mg of protein and represent X ± SD of six different animals.
‘d’ (diacyl), ‘p’ (plasmenyl), and ‘a’ (alkyl) denote the three subclasses (i.e. phosphatidyl-, plasmenyl-, and plasmanyl-), respectively, in the classes of GPCho, GPEtn, and GPSer. DRG, dorsal root ganglia; WT, wild type; ApoE, apolipoprotein E; KO, knockout; DA, presence of a detectable ion peak.