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. 1998 Jun 1;141(5):1107–1119. doi: 10.1083/jcb.141.5.1107

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Characterization of the affinity-purified anti-Sec35p antibody. Extracts were made by glass bead lysis from logarithmically growing strains: wild-type, sec35Δ transformed with pSLY1-20 (CEN), wild-type transformed with pSEC35 (2μm), and sec35-1. All strains were incubated at 21°C before lysis except for the sec35-1 strain, which was maintained at 21°C or shifted to 38°C for 1 h before lysis. Extracts (0.2 OD₅₉₅ per lane) were separated by SDS-12%PAGE and immunoblotted with affinity-purified anti-Sec35p antibodies. The migration of mol wt markers is shown on the right.