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. 1998 Jun 1;141(5):1121–1134. doi: 10.1083/jcb.141.5.1121

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Acidity of multilaminar MHC class II–containing compartments in control Pala B cells and CHS B cells. Cells were incubated with DAMP for 30 min before fixation and processing for cryosectioning. DAMP is visualized on ultrathin cryosections with anti-DNP antibodies, and PAG 5. MHC class II molecules were detected with an anti-class II antibody (PAG 10). The weak base DAMP accumulates in multilaminar MIICs of control cells (A) as well as in the enlarged MIIC in B cells from CHS patients (JH; B). Bars, 200 nm.