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. 1998 Jun 1;141(5):1255–1266. doi: 10.1083/jcb.141.5.1255

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Bcl-2 does not substantially disrupt FADD filaments. HeLa cells were transfected with the expression plasmids indicated and indirect immunofluorescence was performed 48 h posttransfection. HeLa cells were cotransfected with HA-tagged FLICE and Myc-tagged Bcl-2 and then stained with an anti-HA polyclonal antibody (A) or an anti-Myc monoclonal antibody (B). In C, cells were transfected with AU1-tagged FADD alone and visualized with an anti-FADD polyclonal antibody. In D and E, cells were transfected with AU1-tagged FADD and Myc-tagged Bcl-2 and protein expression detected with an anti-FADD polyclonal antibody (D) and an anti-Myc monoclonal antibody (E). In panels F–H, cells were transfected with AU1-tagged FADD, HA-tagged FLICE, and Myc-tagged Bcl-2. Proteins were visualized with anti-FADD polyclonal antibody (F), anti-HA monoclonal antibody (G), and anti-Myc monoclonal antibody (H). All cells were also transfected with CrmA to retain cell viability.