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. 1998 Jun 1;141(5):1169–1179. doi: 10.1083/jcb.141.5.1169

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Spc72p does not bind microtubules in vitro. ³⁵S-labeled in vitro–translated Stu2p and Spc72p were incubated without microtubules (−) and with 5 μM taxol-stabilized bovine brain microtubules (+). Microtubules were then pelleted by centrifugation, and pellet (P) and supernatant (S) fractions were subjected to SDS-PAGE analysis. Bands were visualized and quantitated using a phosphoimager.