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Phenotype of large-budded spc72-2 cells. spc72-2 cells (CUY1123) were grown at 26°C, and were then shifted to 37°C for 2 h. (A–D), DIC; (A′–D′), DAPI staining; (A′′–D′′), immunofluorescence with Tub2p antibody. Arrow indicates microtubule that is unattached to the SPB. Bar, 5 μm.
