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. 1960 Sep 30;112(4):581–594. doi: 10.1084/jem.112.4.581

THE MAMMALIAN CELL-VIRUS RELATIONSHIP

V. SUSCEPTIBILITY AND RESISTANCE OF CELLS IN VITRO TO INFECTION BY COXSACKIE A9 VIRUS

Leroy C McLaren 1, John J Holland 1, Jerome T Syverton 1
PMCID: PMC2137243  PMID: 19867177

Abstract

All primary cell cultures tested from numerous human and rhesus monkey tissues were susceptible to Coxsackie A9 virus infection and were found to contain a specific A9 virus receptor. Ability of these cells to adsorb actively virus correlated with the presence of this receptor. Attachment of A9 virus to cell receptor was specifically enhanced by calcium and magnesium ions. Human cells established in continuous culture from pretested susceptible cultures were found to have lost A9 virus susceptibility and A9 receptors but not receptors for adsorption of type 1 poliovirus or B1, B3, B5 Coxsackie viruses. These cultures were able also to produce infectious A9 virus after exposure to viral RNA. Coxsackie A9 virus as well as type 1 poliovirus, inactivated following reaction with debris prepared from susceptible cells, could be recovered fully in infectious form by treatment of the inactive systems at low pH. Recovery of infectious A9 virus was also possible with a chelating compound. Coxsackie A9 virus could also be dissociated by means of low pH after adsorption onto susceptible cells for 2 minutes at 1°C., but not after 1 hour incubation at 37°C., indicating that viral host cell activity may be required in order for irreversible enterovirus attachment to take place.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER C., KIRBY W. M., WEINSTEIN H. J., YOSHIHARA G. M. Preparation of human amnion tissue cultures. Proc Soc Exp Biol Med. 1956 Jul;92(3):535–538. doi: 10.3181/00379727-92-22535. [DOI] [PubMed] [Google Scholar]
  2. CROWELL R. L., SYVERTON J. T. The viral range in vitro of a malignant, human epithelial cell (strain HeLa, Gey). IV. The cytopathogenicity of C viruses. J Immunol. 1955 Feb;74(2):169–177. [PubMed] [Google Scholar]
  3. DUNNEBACKE T. H., ZITCER E. M. Preparation and cultivation of primary human amnion cells. Cancer Res. 1957 Dec;17(11):1043–1046. [PubMed] [Google Scholar]
  4. EAGLE H., HABEL K., ROWE W. P., HUEBNER R. J. Viral susceptibility of a human carcinoma cell (strain KB). Proc Soc Exp Biol Med. 1956 Mar;91(3):361–364. doi: 10.3181/00379727-91-22263. [DOI] [PubMed] [Google Scholar]
  5. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  6. EVANS A. S. Establishment of human adult tonsil cells in continuous culture and their virus susceptibilities. Proc Soc Exp Biol Med. 1957 Dec;96(3):752–757. doi: 10.3181/00379727-96-23599. [DOI] [PubMed] [Google Scholar]
  7. HOLLAND J. J., McLAREN L. C. Improved method for staining cell monolayers for virus plaque counts. J Bacteriol. 1959 Oct;78:596–597. doi: 10.1128/jb.78.4.596-597.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOLLAND J. J., McLAREN L. C., SYVERTON J. T. The mammalian cell-virus relationship. IV. Infection of naturally insusceptible cells with enterovirus ribonucleic acid. J Exp Med. 1959 Jul 1;110(1):65–80. doi: 10.1084/jem.110.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOLLAND J. J., McLAREN L. C. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959 May 1;109(5):487–504. doi: 10.1084/jem.109.5.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HSIUNG G. D. Effect of kappa-irradiation on patas monkey kidney cell susceptibility to enteroviruses. Virology. 1959 Feb;7(2):140–149. doi: 10.1016/0042-6822(59)90182-5. [DOI] [PubMed] [Google Scholar]
  11. HSIUNG G. D. Use of human kidney cultures in the study of enteroviruses. Proc Soc Exp Biol Med. 1959 Oct-Dec;102:612–616. doi: 10.3181/00379727-102-25335. [DOI] [PubMed] [Google Scholar]
  12. HUMMELER K., KETLER A. Dissociation of poliomyelitis virus from neutralizing antibody. Virology. 1958 Aug;6(1):297–299. doi: 10.1016/0042-6822(58)90082-5. [DOI] [PubMed] [Google Scholar]
  13. LERNER A. M., TAKEMOTO K. K. Human amnion cell cultures; susceptibility to viruses and use in primary virus isolations. Proc Soc Exp Biol Med. 1957 Jan;94(1):179–182. doi: 10.3181/00379727-94-22891. [DOI] [PubMed] [Google Scholar]
  14. LERNER A. M., TAKEMOTO K. K., SHELOKOV A. Human chorion cells: cultivation and susceptibility to viruses. Proc Soc Exp Biol Med. 1957 May;95(1):76–80. doi: 10.3181/00379727-95-23126. [DOI] [PubMed] [Google Scholar]
  15. MANDEL B. Studies on the interactions of poliomyelitis virus, antibody, and host cells in tissue culture system. Virology. 1958 Oct;6(2):424–447. doi: 10.1016/0042-6822(58)90092-8. [DOI] [PubMed] [Google Scholar]
  16. McLAREN L. C., HOLLAND J. J., SYVERTON J. T. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959 May 1;109(5):475–485. doi: 10.1084/jem.109.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PULVERTAFT R. J., DAVIES J. R., WEISS L., WILKINSON J. H. Studies on tissue cultures of human pathological thyroids. J Pathol Bacteriol. 1959 Jan;77(1):19–32. doi: 10.1002/path.1700770103. [DOI] [PubMed] [Google Scholar]
  18. ROBERTSON H. E., BRUNNER K. T., SYVERTON J. T. Propagation in vitro of poliomyelitis viruses. VII. pH change of HeLa cell cultures for assay. Proc Soc Exp Biol Med. 1955 Jan;88(1):119–122. doi: 10.3181/00379727-88-21509. [DOI] [PubMed] [Google Scholar]
  19. SICKLES G. M., MUTTERER M., FEORINO P., PLAGER H. Recently classified types of Coxsackie virus, group A; behavior in tissue culture. Proc Soc Exp Biol Med. 1955 Nov;90(2):529–531. doi: 10.3181/00379727-90-22088. [DOI] [PubMed] [Google Scholar]
  20. SYVERTON J. T., McLAREN L. C. Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver, and lung. Cancer Res. 1957 Oct;17(9):923–926. [PubMed] [Google Scholar]
  21. VOGT M., DULBECCO R. Properties of a HeLa cell culture with increased resistance to poliomyelitis virus. Virology. 1958 Jun;5(3):425–434. doi: 10.1016/0042-6822(58)90037-0. [DOI] [PubMed] [Google Scholar]
  22. ZITCER E. M., DUNNEBACKE T. H. Transformation of cells from the normal human amnion into established strains. Cancer Res. 1957 Dec;17(11):1047–1053. [PubMed] [Google Scholar]

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