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. 1998 Aug 4;95(16):9535–9540. doi: 10.1073/pnas.95.16.9535

Figure 5.

Figure 5

Binding of aged RBC to LOX-1 is accompanied by PS externalization. (a) PS exposure on the surface of aged RBC. RBC (20% hematocrit) were incubated in PBS (solid line) or in PBS containing 0.1% glucose (dashed line) at 37°C for the indicated periods, and binding of annexin V-fluorescein isothiocyanate to RBC was analyzed by flow cytometry. A total of 5,000 cells were analyzed in each condition. (b) Binding of aged RBC to BLOX-1-CHO. RBC were incubated in PBS (○) or in PBS containing 0.1% glucose (•) as described above, and binding of RBC to BLOX-1-CHO was determined. Values represent the number of bound RBC per 100 BLOX-1-CHO cells and are expressed as the mean ± SEM of triplicate determinations.