Figure 3.

Detection of LL-37/hCAP-18 RNA in airway tissue and cultivated respiratory epithelial cells by RT-PCR. Poly(A)⁺ RNA was isolated from human lung (lanes 1 and 2) and cultured airway epithelial cells (lanes 3 and 4) and subjected to RT-PCR by using two different sets of primers specific for LL-37/hCAP-18. The PCR mixtures were fractionated on an agarose gel that was stained with ethidium bromide (Left). DNA from the gel was transferred to a filter, which was hybridized to a LL-37/hCAP-18 probe (Right). An autoradiograph of this experiment is shown. Lanes 1 and 3 represent reactions using the primer combination fall 1 and fall 2, whereas lanes 2 and 4 are reactions using combination fall 1 and fall 8. Molecular weight markers measured as base pairs are shown along the left border.