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. 1998 Aug 4;95(16):9541–9546. doi: 10.1073/pnas.95.16.9541

Figure 4.

Figure 4

Fluorescent in situ hybridization of LL-37/hCAP-18 in proximal human lung. Sections of human lung were hybridized to a LL-37/hCAP-18 RNA probe and immunolabeled by using an antibody to lysozyme as described in Materials and Methods. (A–F) The bronchial surface epithelium, hybridized to the LL-37/hCAP-18 antisense (A) or sense probe (D), stained with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) (B and E) and visualized by Nomarski optics (C and F). (G–L) Specific evaluation of the submucosal glands, showing the hybridization to the LL-37/hCAP-18 antisense (G) or sense probe (J), the colocalization with lysozyme by immunohistochemistry (H and K), and the visualization by Nomarski optics (I and L). Arrows indicate serous cells and arrowheads indicate mucous cells. In each case the magnification is ×100.