Abstract
The antigenicity of an ethylene oxide-treated human serum in humans has been studied. The immune response to the material had many of the characteristics of a delayed cellular skin reaction. Even after repeated immunizations by intradermal skin testing over a period of 1½ years, no detectable antibody could be found in the sera. The antigenicity has been shown to be associated with drastic alteration of the homologous serum proteins as evidenced by (a) the formation of new proteins, and (b) the poor cross-reactions of the modified serum proteins with antisera against normal human serum albumin and normal human gamma globulin. The delayed hypersensitivity was transferable to normal recipients by either viable or killed leukocytes. The implications of these findings have been discussed with respect to the problem of sterilization of sera, the tuberculin reaction, and autoimmune phenomena.
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Selected References
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- BENACERRAF B., GELL P. G. Studies on hypersensitivity. III. The relation between delayed reactivity to the picryl group of conjugates and contact sensitivity. Immunology. 1959 Jul;2:219–229. [PMC free article] [PubMed] [Google Scholar]
- HALBERT S. P., SWICK L., SONN C. The use of precipitin analysis in agar for the study of human streptococcal infections. II. Ouchterlony and Oakley technics. J Exp Med. 1955 May 1;101(5):557–576. doi: 10.1084/jem.101.5.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Macpherson C. F. QUANTITATIVE MICRO-ESTIMATION OF ANTIBODIES IN THE SERA OF MAN AND OTHER ANIMALS. Science. 1943 Apr 30;97(2522):405–406. doi: 10.1126/science.97.2522.405. [DOI] [PubMed] [Google Scholar]
- ISHIZAKA K., ISHIZAKA T. Biologic activity of aggregated gamma-globulin. II. A study of various methods for aggregation and species differences. J Immunol. 1960 Aug;85:163–171. [PubMed] [Google Scholar]
- LAWRENCE H. S., PAPPENHEIMER A. M., Jr Transfer of delayed hypersensitivity to diphtheria toxin in man. J Exp Med. 1956 Sep 1;104(3):321–335. doi: 10.1084/jem.104.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAWRENCE H. S. The cellular transfer of cutaneous hypersensitivity to tuberculin in man. Proc Soc Exp Biol Med. 1949 Aug;71(4):516–522. doi: 10.3181/00379727-71-17242. [DOI] [PubMed] [Google Scholar]
- LAWRENCE H. S. The transfer in humans of delayed skin sensitivity to streptococcal M substance and to tuberculin with disrupted leucocytes. J Clin Invest. 1955 Feb;34(2):219–230. doi: 10.1172/JCI103075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markham R. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J. 1942 Dec;36(10-12):790–791. doi: 10.1042/bj0360790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maurer P. H., Subrahmanyam D. IMMUNOLOGICAL STUDIES WITH PLASMA EXPANDERS DERIVED FROM HUMAN PLASMA. J Clin Invest. 1960 Apr;39(4):698–705. doi: 10.1172/JCI104086. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OVARY Z. Immediate reactions in the skin of experimental animals provoked by antibody-antigen interaction. Prog Allergy. 1958;5:459–508. [PubMed] [Google Scholar]
- RAM J. S., MAURER P. H. Immunochemical studies on some acetylated proteins; acetylated rabbit serum albumin, an antigen in rabbits. Arch Biochem Biophys. 1957 Nov;72(1):119–126. doi: 10.1016/0003-9861(57)90179-0. [DOI] [PubMed] [Google Scholar]
- RAPAPORT F. T., LAWRENCE H. S., MILLAR J. W., PAPPAGIANIS D., SMITH C. E. Transfer of delayed hypersensitivity to coccidioidin in man. J Immunol. 1960 Apr;84:358–367. [PubMed] [Google Scholar]
- SALVIN S. B. Occurrence of delayed hypersensitivity during the development of Arthus type hypersensitivity. J Exp Med. 1958 Jan 1;107(1):109–124. doi: 10.1084/jem.107.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. Delayed hypersensitivity and the anamnestic response. J Immunol. 1960 May;84:449–457. [PubMed] [Google Scholar]
- WARD R., RADER D., LIPTON M. M., FREUND J. Formation of neutralizing antibody in monkeys injected with poliomyelitis virus and adjuvants. Proc Soc Exp Biol Med. 1950 Jul;74(3):536–539. doi: 10.3181/00379727-74-17964. [DOI] [PubMed] [Google Scholar]