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Fig. 1. The effects of fMet-tRNAfMet and 30S subunits on the affinity of GDP or GTP to IF2. The extent of GDP (A) or GTP (B) binding to IF2 was measured by nitrocellulose filtration as a function of G-nucleotide concentration in the presence of combinations of fMet-tRNAfMet and 30S subunits containing mMFTI mRNA and IF1. Open circles: with neither fMet-tRNAfMet nor 30S; closed circles: with 30S; open squares: with fMet-tRNAfMet; closed squares: with fMet-tRNAfMet and 30S.
