Table II.
Quantitation of the Effect of Competitors of Fibronectin–Integrin Interactions on the Cohesion of Neural Crest Cells
| Inhibitor of cell–substratum adhesion | Concentration | Number of particles | ||
|---|---|---|---|---|
| μg/ml | ||||
| None | / | 230 ± 20 | ||
| RGDS | 100 | 165 ± 19 | ||
| 500 | 78 ± 5 | |||
| 1,000 | 50 ± 2 | |||
| 2,000 | 53 ± 7 | |||
| GRDGS | 1,000 | 215 ± 10 | ||
| GPenGRGDSPCA | 100 | 137 ± 24 | ||
| 250 | 99 ± 14 | |||
| 500 | 58 ± 14 | |||
| 1,000 | 64 ± 18 | |||
| mAb 333 anti-fibronectin | 5 | 129 ± 60 | ||
| 50 | 56 ± 14 | |||
| Ab 2992 anti-β1 integrin | 100 | 113 ± 12 | ||
| 1,000 | 42 ± 5 | |||
| mAb CSAT anti-β1 integrin | 10 | 162 ± 25 | ||
| 20 | 54 ± 9 | |||
| 40 | 44 ± 10 | |||
| mAb ES66-8 anti-β1 integrin | 100 | 215 ± 10 | ||
| mAb HP2/1 anti-α4 integrin | 10 | 138 ± 22 | ||
| 20 | 80 ± 24 | |||
| 40 | 68 ± 15 | |||
| mAb B5G10 anti-α4 integrin | 50 | 220 ± 25 | ||
| mAb LM609 anti-αVβ3 integrin | 1/250* | 203 ± 16 | ||
| 1/100* | 88 ± 38 | |||
| 1/25* | 67 ± 11 |
Results are expressed as the number of particles that were counted in an arbitrarily defined region of each neural crest outgrowth and covering ∼0.2 mm2, after 5 h of incubation with the competitor. Values represent the mean ± SD of a minimum of eight measurements in at least two independent experiments.
*
Dilution of ascitic fluid.