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. 1997 Jun 30;137(7):1639–1649. doi: 10.1083/jcb.137.7.1639

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Localization of ZAP-70 is unaffected by TCR stimulation. Jurkat T cells were either unstimulated (A), or stimulated for 5 min with F(ab)₂ fragments of the CD3 mAb, OKT3 (B), fixed, and double stained for endogenous ZAP-70 (left) and total phosphotyrosine (right). Staining for endogenous ZAP-70 with ZAP-4 antiserum also produced cytoplasmic fluorescence which was nonspecific (Fig. 1). Bar, 10 μm.