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. 1997 Jun 30;137(7):1639–1649. doi: 10.1083/jcb.137.7.1639

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ZAP-70 requires an active kinase domain for its localization. Jurkat T cells (A–D) or NIH 3T3 fibroblasts (E–H) were transfected with plasmids encoding either: wild-type ZAP-70–HA (A and E), ΔSH2-ZAP-70–HA (B and F), ΔSph-ZAP-70–HA (C and G), or kinase-dead ZAP-70.K369A–HA. Transfected cells were stained with 12CA5 to reveal the HA-tagged ZAP-70 protein. Bars, 10 μm.