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. 1997 Jun 30;137(7):1469–1482. doi: 10.1083/jcb.137.7.1469

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In sec13-1 mutants, Gap1p exits the ER but is not transported to the plasma membrane. (A) Wild-type (CKY465) and sec13-1 (CKY466) strains expressing Gap1p-HA were grown in ammonia medium at 24°C, and cell lysates were fractionated on density gradients of 20–60% sucrose containing 10 mM EDTA. Gap1p-HA and markers for different subcellular compartments were evaluated for each fraction as described in Fig. 3. (B) Wild-type (CKY465) and sec13-1 (CKY466) strains expressing Gap1p-HA were grown in ammonia medium at 24°C, and cell lysates were fractionated on density gradients of 20–60% sucrose containing 1 mM Mg²⁺.