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. 1997 Jun 30;137(7):1581–1588. doi: 10.1083/jcb.137.7.1581

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Bcl-2 expression allowed sod1Δ mutants to form colonies in 100% oxygen. sod1Δ (EG118), sod2Δ (EG110), and sod1Δsod2Δ (EG133) cells harboring pAD4–bcl-2 (crosshatched bars) or pAD4 (open bars), were grown to stationary phase and equal numbers of cells were plated on selective plates (SD–leu) and incubated in low aeration (CampyPaks) for 3 d, or in an atmosphere of 100% oxygen for 48 h followed by incubation in air. (Oxygen was replenished after 24 h.) Viability was recorded when colonies became big enough to count—at day 3 (EG118) or day 4 (EG110 and EG133)—and is reported as a percentage of the viability of the same strain in low aeration (relative viability). The experiment was performed with similar results at least four times for each strain, with two separate samples in each experiment. A representative experiment is shown.