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. 1997 Jun 30;137(7):1483–1493. doi: 10.1083/jcb.137.7.1483

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The association of BiP and the Sec63p lumenal domain depends on the presence of hydrolyzable ATP. Binding reactions using 63Jp glutathione agarose beads were performed as in Fig. 3. (A) Final concentration of nucleotide in the reaction is indicated. The entire binding reaction depicted in lane 8 was done in the absence of Mg²⁺ plus 10 mM EDTA. (B) Bound nucleotides associated with glutathione beads (bound to either 63Jp or GST as indicated above the lanes) in the presence (+) or absence (−) of BiP after washing the beads were visualized by thin layer chromatography and a Phosphorimager.

The association of BiP and the Sec63p lumenal domain depends on the presence of hydrolyzable ATP. Binding reactions using 63Jp glutathione agarose beads were performed as in Fig. 3. (A) Final concentration of nucleotide in the reaction is indicated. The entire binding reaction depicted in lane 8 was done in the absence of Mg²⁺ plus 10 mM EDTA. (B) Bound nucleotides associated with glutathione beads (bound to either 63Jp or GST as indicated above the lanes) in the presence (+) or absence (−) of BiP after washing the beads were visualized by thin layer chromatography and a Phosphorimager.