Table IV.
Variation of kMt Numbers with the Stage of Mitosis and Taxol Treatment
Mono- oriented | Late Pro-metaphase* | Mature metaphase‡ | Early anaphase§ | Taxol (10 min)‖ | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Average | 11.9 | 19.7 | 24.3 | 27.8 | 28.7 | |||||
Standard deviation | 5.9 | 6.7 | 4.9 | 6.3 | 7.4 | |||||
Range | 3–27 | 7–34 | 11–35 | 14–44 | 12–52 | |||||
n | 30 | 94 | 62 | 65 | 54 |
Late prometaphase chromosomes came from cells with 1–4 monooriented chromosomes.
Mature metaphase chromosomes came from cells filmed for 20–21 min after the last monooriented chromosome initiated congression.
Early anaphase chromosomes came from cells fixed 1–3 min after chromatid separation.
Metaphase cells (all chromosomes at the spindle equator) were treated for 10 min with 1.0 μM taxol before fixation.