Figure 7.
SED5 overexpression suppresses vti1Δ lethality. Growth of the GAL1–VTI1 strain alone or overexpressing Sed5p or Pep12p on plates containing galactose (Gal) or glucose (Glc). The GAL1–VTI1 strain could not grow on glucose after the expression of VTI1 was shut off. Overexpression of the early Golgi t-SNARE Sed5p allowed growth in the absence of Vti1p. Overexpression of the prevacuolar-localized t-SNARE Pep12p did not support growth of vti1Δ cells.