Table II.
Plasmids Used in this Study
| Plasmid | Description | Reference | ||
|---|---|---|---|---|
| pSN206 | ADH1-LexA-VPS10(tail) fusion in pEG202 (2μ-HIS3) | This study | ||
| p2H-1 | 5 kb genomic DNA with VTI1 (codons 106-217) in pGAD3 | This study | ||
| pSN258 | vti1Δ::HIS3 disruption construct in pRS305 | This study | ||
| pFvM7 | E. coli expression vector pGex2T encoding GST-Vtilp (aal-194) | This study | ||
| pFvM16 | GAL1-VTI1::HA in pRS314 (CEN6-TRP1) | This study | ||
| pFvM17 | GAL1-VTI1 in pRS314 (CEN6-TRP1) | This study | ||
| pFvM20 | E. coli expression vector pMal-p2 encoding MBP-Vtilp::HA (aal-194) | This study | ||
| pFvM28 | 1.8 kb containing VTI1 in pRS314 (CEN6-TRP1) | This study | ||
| pFvM29 | 1.8 kb containing VTI1 in pRS316 (CEN6-URA3) | This study | ||
| pFvM32 | 1.8 kb containing VTI1 in YEp352 (2μ-URA3) | This study | ||
| pFvM38 | E. coli expression vector pQE30 encoding 6His-Vtilp (aal-194) | This study | ||
| pFvM40 | E. coli expression vector pGEX2T encoding GST-Sed5p (aa1-324) | This study | ||
| pFvM47 | 7.7 kb Chrom XII (nt190000-197700) containing SED5 in YEp24 (2μ-URA3) | This study | ||
| pFvM89 | SED5 in YEp351 (2μ-LEU2) | This study | ||
| pFvM92 | vti1-11 in pRS314 (CEN6-TRP1) | This study | ||
| pFvM93 | vti1-2 in pRS314 (CEN6-TRP1) | This study | ||
| pFvM103 | E. coli expression vector pGex2T encoding GST-Sso2p (aal-269) | This study | ||
| pFvM104 | 2.9 kb EcoRI-SnaBI DNA from pRB58 with SUC2 in pRS316 | This study | ||
| pRCP59 | E. coli expression vector pGex3X encoding GST-Pep12p (aal-263) | R.C. Piper*/T.H. Stevens | ||
| pRCP110 | PEP12 in YEp351 (2μ-LEU2) | R.C. Piper*/T.H. Stevens |
*
Institute of Molecular Biology, University of Oregon, Eugene, OR