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. 1997 Jun 30;137(7):1555–1566. doi: 10.1083/jcb.137.7.1555

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A 190-kD putative actin-binding protein is present in bovine adrenal tissue. MFs assembled from purified rabbit muscle actin, or MTs assembled with taxol from purified bovine brain tubulin, were added to bovine adrenal cytosol at 4°C. After centrifugation, the MT and MF pellets were resuspended and analyzed by SDS-PAGE. As can be seen, a prominent 190-kD polypeptide was present exclusively in the MF pellet.