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. 1997 Jun 30;137(7):1555–1566. doi: 10.1083/jcb.137.7.1555

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IQGAP1 colocalizes with cytochalasin D–sensitive microfilaments in lamellipodia and ruffles, but not in stress fibers. CV-1 cells were stained with a rabbit antibody to IQGAP1 (17) followed by rhodamine-labeled goat anti–rabbit IgG and Bodipy–phallicidin (to visualize F-actin). Intense IQGAP1 and F-actin staining was colocalized in lamellipodia located at cell margins (top row), and in ruffles located on the upper surfaces of some cells (middle row). Weak, diffuse staining by anti-IQGAP1 was also seen throughout the cytoplasm, and may represent soluble protein. Stress fibers, which contain densely packed MFs and were brightly stained by Bodipy–phallicidin, were not stained by anti-IQGAP1. After a 30-min exposure to 5 μg/ml cytochalasin D, which caused F-actin to depolymerize, cells were substantially depleted of IQGAP1-positive structures, and of stress fibers as well (bottom row). Bar, 10 μm.