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. 1997 Jun 30;137(7):1615–1626. doi: 10.1083/jcb.137.7.1615

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Targeted disruption of the MAP1B gene in ES cells and mice. (A) Targeting strategy: Wild-type allele containing the first coding exon of the MAP1B gene, replacement-type targeting vector, and targeted allele after homologous recombination are shown. P1, primer 1; P2, primer 2; P3, primer 3; B, BamHI; E, EcoRI; H, HindIII. Bar, 1 kb. (B) Genotype of F2 mice of R21 line. Tail DNA was isolated and analyzed by Southern blotting using the 5′-flanking probe (A, probe) after digestion with BamHI. Lane 1, MAP1B−/− mouse; lane 2, MAP1B+/− mouse; lane 3, MAP1B+/+ mouse. WT, wild-type band; HR, homologous recombinant band.