Fig. 2. A yeast mutant lacking the N-terminal 50 residues of Tim23. (A) Growth of tim23-3 mutant cells is indistinguishable from wild-type cells. Wild-type (WT) and tim23-3 cells were subjected to consecutive 10-fold dilutions, spotted on fermentable medium (YPD) or non-fermentable medium (YPG) and grown for 3 days at the indicated temperatures. (B) Protein composition of tim23-3 mitochondria. Wild-type mitochondria and tim23-3 mitochondria were subjected to SDS–PAGE and immunodecoration. Odd-numbered and even-numbered samples, 10 and 20 µg mitochondrial protein, respectively. (C) BN–PAGE of digitonin-lyzed wild-type and tim23-3 mitochondria and immunodecoration.