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. 2007 Mar 19;204(3):481–487. doi: 10.1084/jem.20061791

Figure 2.

Figure 2.

Enhanced S. mansoni egg–induced type 2 inflammation in the lungs of IL-31Rα−/− mice. (A and B) Paraffin sections of lungs from egg-injected mice at day 14 after injection were H &E stained and the area of inflammation surrounding WT (open bar) and IL-31Rα−/− (closed bar) S. mansoni egg-induced granulomas was measured using OpenLab software. Mean ± SEM; n = 40 granulomas per group. (C) Draining mediastinal lymph node cells from egg-injected WT and IL-31Rα−/− mice were stimulated with either media or 20 μg/ml S. mansoni egg antigen (SEA) for 48 h. Supernatants were analyzed by ELISA for IL-4, -5, and -13. Mean ± SEM of replicate cultures. (D) Serum levels of IgE from WT and IL-31Rα−/− mice were measured by ELISA. Mean ± SEM; n = 3 mice per group. (E) Immunofluorescence staining for RELM-α (red) and counterstain for DAPI (blue). (F) Collagen deposition in the lungs of WT and IL-31Rα−/− mice was detected by Masson's trichrome staining; blue staining demarks collagen. Results are representative of three independent experiments of three mice per group. Asterisk indicates statistically significant as determined by two-tailed Student's t test (P < 0.05). Bars, 60 μm.