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. 2007 Mar 19;204(3):533–545. doi: 10.1084/jem.20062006

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Mouse and human MTP exhibit different mechanisms of transcriptional regulation. (A) Wild-type mouse splenocytes were stimulated for 24 h with the indicated compounds. Total RNA was harvested, and 1 μg was used for cDNA synthesis. MTP (left) and IL-2 (right) transcript levels were quantified by real-time PCR and adjusted to 18S rRNA levels. Both mttp and mttp_v1 are amplified in this assay. (B) Human PBMCs were stimulated for 1, 3, or 5 d with the indicated mitogens. MTP (left) and IL-2 (right) transcript levels were quantified by real-time PCR as in A. (C) Human PBMCs stimulated for 3 d with the indicated mitogens were stained with anti-CD3 and anti-CD69 and analyzed by flow cytometry. (D) HepG2 cells and human PBMCs were stimulated for 3 d with the indicated compounds. MTP transcript levels, quantified by real-time PCR as in A, are shown as the fold change compared with unstimulated cells. Values are ±SD.