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. 2007 Mar 19;204(3):533–545. doi: 10.1084/jem.20062006

Figure 5.

Figure 5.

MTP and MTPv1 directly transfer phospholipid to CD1d in vitro. (A) 2 μg BSA or recombinant mouse CD1d, produced in a baculovirus expression system, were coated onto a microtiter plate. Vesicles containing NBD-labeled PE or triglyceride were added with 0.1 μg of purified MTP-FLAG or MTPv1-FLAG, as indicated, for 2.5 h. After washing, isopropanol was added to each well, and the total fluorescence was measured. Values shown are total fluorescence minus background. *, P < 0.05; and **, P < 0.01 versus no MTP. (B) Lipid transfer assays were performed as in A except that microtiter wells were coated with protein G, protein G plus mouse CD1d-Fc, or protein G plus mouse MHC I-Fc. *, P < 0.05 versus transfer to protein G and P < 0.005 versus transfer to MHCI-Fc. (C) Lipid transfer assays were performed as in A, except that microtiter wells were coated with protein G or protein G plus human CD1d-Fc. *, P < 0.002 versus no MTP. Values are ±SD.