Table II.
CD103 levels reflect T cell clone antitumor cytolytic activity
CD103 expression
|
% of lysis
|
|||
---|---|---|---|---|
% | MFI | Medium | Anti-CD103 | |
PBL | ||||
H32-22 | 3a | 9b | 7 | |
H32-8 | 85 | 81 | 32 | 6 |
H32-25 | 72 | 119 | 49 | 21 |
TIL | ||||
Heu171 | 95 | 293 | 60 | 8 |
Immunofluorescence and cytotoxic activity of T cell clones. Data shown are representative of three independent experiments.
Percentages of CD103+ T cells and mean fluorescence intensities (MFIs) are indicated.
Cytotoxicity toward autologous IGR-Heu tumor cell line was determined by a conventional 4 h 51Cr-release assay at a 20:1 E/T cell ratio.