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. 2007 Mar 19;204(3):667–680. doi: 10.1084/jem.20061287

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — TRAIL receptor expression on hepatocytes in HBV infection. (a) Paraffin-embedded sections from HBV-infected (left) and healthy control (right) livers were stained with an anti–TRAIL-R2 mAb. Membrane-localized (arrows) and cytoplasmic (*) TRAIL-R2 expression is indicated by the brown chromogen reactivity. Bars: top, 40 μm; bottom, 16 μm. (b) Mean fluorescence intensity of HepG2 TRAIL-R2 levels after IL-8 incubation (10 ng/ml for 24 h) compared with untreated and isotype controls. (c) Mean fluorescence intensity of HepG2 TRAIL-R4 levels after 1,000 U/ml IFN-α for 24 h compared with untreated and isotype controls. These are representative of five separate experiments.