Fig. 4. High molecular weight proteins associate with the msl-2 3′UTR in a dSXL-dependent manner. The ³²P-labelled msl-2 3′probe was incubated in a typical translation reaction in the absence (lanes 1–5) or presence (lanes 6–10) of recombinant dSXL. After UV-crosslinking and digestion with RNase T1, complexes were immunoprecipitated with α-SXL antibody or pre-immune serum (ctrl). T, total set of crosslinked proteins before immunoprecipitation; S, supernatant after immunoprecipitation; P, pellet. The position of crosslinked recombinant dSXL is indicated. Asterisks mark proteins that crosslink consistently, bands marked with a dot are not always seen.