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. 1997 Aug 25;138(4):891–899. doi: 10.1083/jcb.138.4.891

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Coinjection of the full-length (504) occludin mRNA with the most COOH-terminally truncated occludin mRNA (266) rescued the tight junction leakage caused by injection of construct 266 alone. The experimental procedure was same as in Fig. 3. The frozen sections from the embryos injected with ∼2 pg of 504 (a), 266 (b), or 504 and 266 (c) construct mRNA were stained with RITC-avidin. The extraembryonic space is toward the top of figure in a, and left in b and c. No leak was seen in the embryo infected with 504 mRNA (a). Biotin molecules clearly penetrated through the tight junctions and labeled the intercellular spaces between the apical cells of embryo injected with 266 mRNA (b). However, there was no detectable leak in the embryo injected with both 504 and 266 mRNA (c). Bar, 15 μm.