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Double labeling of frozen sections of Xenopus embryos with anti-occludin 11350 (a) and anti-FLAG M2 (b). 4 nl (∼10 pg) of mutant 266 mRNA were microinjected into the anterior, dorsal blastomere of eight-cell stage embryo and incubated for 8 h at room temperature before freezing. The arrowheads indicate the colocalization of Xenopus full-length occludin and chicken truncated occludin at the junctional complexes. Bar, 10 μm.
