Figure 6.

Src rescues transformed fibroblasts from apoptosis triggered by the loss of ECM adhesion. Three separately grown clones were isolated from REF cultures transfected with either E1A and Src or Myc and Src (filled bars). 10⁵ cells from each clone were plated onto polyHEMA-coated TCP (HEMA), regular TCP dishes (TCP), or regular TCP dishes and irradiated after 24 h with 500 cGy ionizing radiation (5Gy XRT). Viability was determined by trypan blue 24 h after plating onto TCP or polyHEMA and 24 h after radiation for XRT controls. Bars represent the average of three experiments (±SD).