Figure 6.

Moesin copurifies with GTPγS-dependent biological activity in permeabilized cells. (A) Silver-stained 10% SDS-PAGE using 10-μl aliquots from fractions 28–35 of the gel-filtration column shown in Fig. 5 D. Relative activities of the gel-filtered fractions are noted below the corresponding lanes of the blot. (B) 22 μg of fraction 22, the most active from the Q-Sepharose column shown in Fig. 5 c, was electrophoresed on 10% SDS-PAGE and electroblotted onto PVDF, and the major protein bands A–F were excised and microsequenced. The sequences obtained are shown. (C) Proteins from a parallel gel to A were transferred to PVDF and Western blotted with a polyclonal anti-ERM antibody.