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. 1997 Aug 25;138(4):799–810. doi: 10.1083/jcb.138.4.799

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Expression of myc epitope-tagged domains verified by Western analysis. (A) The endogenous 76-kD ORF1 and 160-kD full length kelch proteins are detected in wild-type (w¹¹¹⁸) egg chambers; both proteins are absent in kel^DE1 egg chambers. An ∼80-kD myc:ORF1 product is detected in kel^DE1 egg chambers. A 65-kD myc:ΔNORF1 (two representative lines, 14-1 and 15-2, are shown), a 47-kD myc:ORF1-R, a 35-kD myc:BTBIVR, and a 17-kD myc:BTB protein are detected in wild-type egg chambers by anti-kelch 1B antibodies. (B) A 33-kD myc:KREP product is detected in one transgenic line with unpurified anti-KREP polyclonal antibodies. Endogenous kelch proteins are also detected, although the ORF1 product is significantly obscured by a prominent group of nonkelch proteins with which the polyclonal antibodies cross-react. These cross-reacting proteins are also detected in kel^DE1extracts. Only the expected 33-kD KREP protein is unique to the myc:KREP line.

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