Figure 5.

Myc:ΔNORF1 expressed at a low level can rescue kel^DE1 mutants. (A) Myc:ΔNORF1 15-2 expresses myc: ΔNORF1 at a lower level and localizes to ring canals of a wild-type stage 10 egg chamber. (B) The same egg chamber as in A stained for actin shows intact nurse cell membranes and many intact ring canals. (C) The same line myc:ΔNORF1 15-2 in a wild-type background expressed myc: ΔNORF1 that localized to region 2b ring canals (arrow), which is earlier than normal, but the protein was not as abundant. (D) Actin staining of the same germarium as in C also showed actin localization to ring canals (arrow) at the same time as myc:ΔNORF1. (E) Myc:ΔNORF1 15-2 in a stage 9 kel^DE1 egg chamber rescued the ring canals and egg chamber morphology. Myc:ΔNORF1 protein localized to ring canals normally. (F) Actin staining of the same egg chamber in E revealed intact nurse cell membranes and ring canals with a robust, well organized actin inner rim. A, C, and E were stained with anti-myc 9E10 antibodies. Bars, 30 μm.