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. 1997 Aug 25;138(4):877–889. doi: 10.1083/jcb.138.4.877

Figure 9.

Figure 9

Effect of filipin on the endocytosis of LDL by brain capillary ECs. (A) The cells were pretreated with filipin (3 μg/ml) before the addition of DiI-LDL, and the endocytosis was performed as described in the Fig. 8, legend. (B) Immediately after filipin treatment, the cells were incubated in DME containing 20% CS for 30 min to reverse the effects of filipin before examining DiI-LDL endocytosis. (C and D) Endocytosis was carried out with brain capillary ECs in growing phase without (C) or with (D) pretreatment with filipin. Bar, 50 μm.