Table I.
Effects of Overexpression on Ca2+ Signals
| Clone | Fold overexpression* | Ca2+ (nM)‡ | ||||||
|---|---|---|---|---|---|---|---|---|
| PDGF | Bradykinin | |||||||
| Cap G | ctrl | 1 | 232 ± 30 | 659 ± 95 | ||||
| gK6 | 1.3 | — | 259 ± 58 | |||||
| gK8 | 1.4 | 323 ± 14 | 196 ± 21 | |||||
| gK15 | 1.5 | 339 ± 40 | 151 ± 57 | |||||
| Gelsolin | Ctrl | 1 | 289 ± 38 | 790 ± 146 | ||||
| C3 | 1.3 | 352 ± 20 | 1358 ± 251 | |||||
| C6 | 1.4 | 179 ± 25 | 270 ± 60 | |||||
| C1 | 1.7 | 125 ± 38 | 80 ± 18 | |||||
| C4 | 2.1 | 104 ± 10 | 30 ± 5 | |||||
| C5 | 2.4 | 125 ± 15 | 20 ± 2 | |||||
*
Gelsolin content was determined by scanning of Western blots, using mouse and human gelsolin as standards to quantitate overexpressed and endogenous gelsolins, respectively. Values were the means of three determinations. Cap G content was determined as described in reference 31.
‡
Peak Ca2+ concentration after stimulation with 50 ng/ml PDGF or 5 μM bradykinin. Values were the means ± SEM of three to five determinations.