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Cooperativity of cofilin binding to rabbit muscle F-actin at pH 6.5. 6 μM F-actin was cosedimented with cofilin (0–35 μM). Supernatant and pellets were analyzed by SDS-PAGE and concentrations of cofilin and actin estimated by densitometry. Bound cofilin is expressed as a molar ratio to actin subunits. Nonlinear least-squares fitting shows a maximum binding at 1.16 cofilin/actin and a Hill constant of 6.4.
