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. 1997 Aug 25;138(4):861–875. doi: 10.1083/jcb.138.4.861

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Syntaxin1 levels during embryogenesis. Protein was extracted from timed embryo collections of the ages indicated (hours AED) and from adult heads and separated by SDS-PAGE. Per lane, the protein extracted from 30 embryos or one half of an adult head was loaded. The gels were blotted onto Immobilon membranes and were then probed with the monoclonal antibody 8C3. Syntaxin1 is present in 0–3-h embryos, the stage during which cellularization occurs, and levels increase as the nervous system matures. In both embryos and adults, the protein has a molecular mass of 35 kD, as predicted.