Figure 2.

M-C₆-NBD-PE is sorted to the mitochondria and nuclear envelope from the yeast plasma membrane. CRY3 was grown to mid-log phase in SDC medium at 30°C and incubated with M-C₆-NBD-PE–containing vesicles for 1 h as described in Materials and Methods. Colocalization of NBD fluorescence with DAPI fluorescence of nuclear and mitochondrial DNA was performed by including 2.5 μg/ml DAPI with the M-C₆-NBD-PE–containing vesicles. Top row, NBD fluorescence; middle row, DAPI fluorescence; bottom row, DIC optics. Solid arrows point to nuclear envelope (NBD fluorescence) and nuclear DNA (DAPI fluorescence); open arrows point to mitochondria (NBD fluorescence) and mitochondrial DNA (DAPI fluorescence). Bar, 10 μm.