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. 1997 Jul 28;138(2):255–270. doi: 10.1083/jcb.138.2.255

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tpe mutants are inhibited in the accumulation of M-C₆-NBD-PE. TPE1-1 and its Tpe⁺ parent strain, CRY2, were grown to mid-log phase at 23°C. The incubation temperature was then shifted to 37°C for 30 min, and M-C₆-NBD-PE internalization assays were performed for 1 h before microscopic analysis as described in Materials and Methods. (A–C) CRY2. (D–F) TPE1-1. (G–I) tpe2-1. (A, B, D, E, G, and H) NBD fluorescence. (C, F, and I) DIC optics. In A and D, a neutral density filter was used that attenuated the excitatory light by 95%. In B and E, the filter was removed to allow 100% of the excitatory light to impinge upon the sample.