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. 1997 Jul 28;138(2):255–270. doi: 10.1083/jcb.138.2.255

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TPE1-1 and tpe2-1 display temperature-sensitive growth defects on nonfermentable carbon sources. TPE1-1, tpe2-1, and CRY2 were plated on rich media containing both a fermentable carbon source (2% glucose) (YPD), or the nonfermentable carbon sources glycerol (2%) and ethanol (2%) (YEP-GE). Plates were incubated for 2 d (30° and 37°C plates) or 4 d (23°C) before photography.