Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Jul 28;138(2):255–270. doi: 10.1083/jcb.138.2.255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

The inhibition of M-C₆-NBD-PE accumulation by mutations in PDR3 is allele specific. pdr3-2 and tpe2-1/pdr3-11 strains were grown to mid-log phase in SDC medium at 23°C. 1 ml of each culture was incubated at 37°C for 30 min and M-C₆-NBD-PE internalization assays and fluorescence microscopy were performed as described in Materials and Methods. (A–C) tpe2-1/pdr3-11. (D–F) pdr3-2. (A, B, D, and E) NBD fluorescence. (C and F) DIC optics. In A and D, a neutral density filter was used that attenuated the excitatory light by 95%. In B and E, the neutral density filter was removed to allow 100% of the excitatory light to reach the samples.