Table III.

Internalization of NBD-labeled Lipids and FM4-64 into Mutant and Wild-Type Yeast Strains

		Strains
		CRY2		TPE1-1/PDR1-11		tpe2-1/pdr3-11
A M-C6-NBD-PE		100 ± 16		1 ± 1		0.3 ± 0.3
B M-C6-NBD-PC		100 ± 38		12 ± 3		18 ± 8
C FM4-64		100 ± 58		35 ± 23		36 ± 24

Yeast strains were grown to mid-log phase, and lipid internalization assays, microscopy, and pixel brightness analysis were perfomed as described in Materials and Methods. For each determination, the mean pixel brightness of 10–38 cells and standard deviation were determined. For each internalization condition, the pixel brightness of CRY2 was set to 100%, and the pixel brightness in the other strains is shown as a percentage of this value. The pixel brightness of CRY2 under the various incubation conditions was: (A) 177 ± 28; (B) 132 ± 50; (C) 12 ± 7.