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. 1997 Jul 28;138(2):423–434. doi: 10.1083/jcb.138.2.423

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Protein levels of endogenous and transfected ezrin. Serial dilutions of total cell extracts (0.1–2 μg) were run on a 7% SDS-PAGE under reducing conditions, and transferred to nitrocellulose membranes. The membranes were probed with polyclonal anti-ezrin antibody (lanes A–C) and monoclonal P5D4 antibody (lane D), followed by peroxidase-conjugated secondary antibody, and developed with chemiluminescence. (Lanes A–D) 1 μg of total cell extract. (Lane A) LLC-PK1 cells; (lane B) E7 cells; (lane C) FF1 cells (see p. 428); (lane D) N2 cells.

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