Table I.

Mitogenic Effect of HGF on LLC-PK1 Cells

		LLC-PK1		E7 Clone		N2 Clone
1. −HGF		88.2 ± 16.0		88.8 ± 5.6		90.0 ± 7.9
2. +HGF		149.8 ± 18.0		150.0 ± 22.0		138.0 ± 12.4

4 × 10⁴ cells were seeded in 96-well microtiter plate to reach confluence, starved in DME 0.2% FCS for 24 h, and then stimulated with 15 ng/ml of HGF. After 6 h, BrdU was added and left to incorporate for an additional 3 h. Incorporated BrdU was detected by a specific mAb and peroxidase-conjugated secondary antibody. After incubation with the peroxydase substrate, absorbance was read at 410 nm. Values are given as OD arbitrary units. Numbers are the mean (± SD) calculated from six duplicates of one representative experiment. The t test was applied for specific comparisons of the means of two pairs of samples in each column and in each row. Comparisons between HGF-treated and untreated cells samples (1 vs 2) gave P ⩽ 0.01, while comparisons between samples of each row did not give significant P.