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. 1997 Jul 28;138(2):395–409. doi: 10.1083/jcb.138.2.395

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KLP38B binds PP1 in vitro. (Lane 1) Reticulocyte lysate containing ³⁵S-labeled procollagen. This protein was incubated with PP1 beads or control beads. (Lane 2) Eluate from control beads; (lane 3) eluate from PP1 beads. Procollagen does not bind to PP1. (Lane 4) Reticulocyte lysate containing ³⁵S-labeled KLP38B. (Lane 5) Eluate from control beads; (lane 6) eluate from PP1 beads. KLP38B binds to PP1 but not to the control beads. Details of the beads and the binding, washing, and elution conditions are described in Materials and Methods.