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. 1997 Jul 28;138(2):239–253. doi: 10.1083/jcb.138.2.239

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Localization of Xlrbpa in nuclear spreads of Xenopus GVs. (a and b) Lampbrush chromosome preparations were stained with preimmune serum Rb6 or (c and d) immune serum Rb6. Antibodies were detected with a secondary FITC-labeled antibody. (a and c) Phase contrast; (b and d) fluorescence image. (a) Lampbrush chromosomes, C snurposomes (C), B snurposomes (B), and nucleoli (N) can be seen in the phase contrast image. The large C snurposome (C) has a smaller B snurposome attached to its surface. (b) No staining can be observed with preimmune serum Rb6. (c) Nuclear organelles are labeled as in (a). The large C snurposome (C) has an attached B snurposome (B). (d) Essentially all nuclear structures are labeled after staining with serum Rb6. Lampbrush chromosomes, nucleoli (N), C snurposomes (C), and B snurposomes (B) are easily detectable. C snurposomes label most brilliantly while the other structures show only moderate staining with this antibody. A comparison of B and C snurposome staining can easily be made on the large, intensely labeled C snurposome (C) which has a moderately stained, smaller B snurposome on its surface. Bar, 10 μm.